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PDK1 (phospho Ser241) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60222

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human PDK1 around the phosphorylation site of Ser241. AA range:210-259

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    PDPK1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PDK1 (Phospho-Ser241) Antibody. The picture on the right is blocked with the phospho peptide.

  • Background:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.,PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.,subunit:Interacts with TUSC4.,tissue specificity:Appears to be expressed ubiquitously.,

  • Protein Name:

    3-phosphoinositide-dependent protein kinase 1

  • Human Gene Id:

    5170

  • Human Swiss Prot No:

    O15530

  • Mouse Gene Id:

    18607

  • Mouse Swiss Prot No:

    Q9Z2A0

  • Rat Gene Id:

    81745

  • Rat Swiss Prot No:

    O55173

  • Specificity:

    Phospho-PDK1 (S241) Polyclonal Antibody detects endogenous levels of PDK1 protein only when phosphorylated at S241.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1

  • MolecularWeight(Da):

    63152

  • Observed Band(KD):

    63

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.,PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.,subunit:In

  • Subcellular Location:

    intracellular,nucleoplasm,cytoplasm,cytosol,plasma membrane,focal adhesion,postsynaptic density,membrane,cytoplasmic, membrane-bounded vesicle,cell projection,perikaryon,

  • Expression:

    Brain,Coronary artery,Embryonic kidney,Epithelium,Kidney,My,

  • Western blot analysis of lysates from HeLa cells and NIH/3T3 cells, using PDK1 (Phospho-Ser241) Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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