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Paxillin (phospho Tyr31) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60220

  • Amount:

    100ul

  • Molecular Weight:

    64533Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Paxillin around the phosphorylation site of Tyr31. AA range:15-64

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-Paxillin (Y31) Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    PXN

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

  • Background:

    This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011],

  • Protein Name:

    Paxillin

  • Human Gene Id:

    5829

  • Human Swiss Prot No:

    P49023

  • Mouse Gene Id:

    19303

  • Mouse Swiss Prot No:

    Q8VI36

  • Rat Gene Id:

    360820

  • Rat Swiss Prot No:

    Q66H76

  • Specificity:

    Phospho-Paxillin (Y31) Polyclonal Antibody detects endogenous levels of Paxillin protein only when phosphorylated at Y31.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PXN; Paxillin

  • Observed Band(KD):

    68

  • Function:

    function:Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).,PTM:Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.,similarity:Belongs to the paxillin family.,similarity:Contains 3 LIM zinc-binding domains.,similarity:Contains 4 LIM zinc-binding domains.,subunit:Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 (By similarity). Binds ASAP2. Int

  • Subcellular Location:

    Cytoplasm, cytoskeleton . Cell junction, focal adhesion . Cytoplasm, cell cortex . Colocalizes with integrins at the cell periphery. Colocalize with PXN to membrane ruffles and the leading edge of migrating cells (PubMed:23128389). .

  • Expression:

    Brain,Epithelium,Lung,Placenta,T-cell,Uterus,

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