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NOS1 (phospho Ser852) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60195

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human n-NOS around the phosphorylation site of Ser852. AA range:818-867

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    NOS1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using n-NOS (Phospho-Ser852) Antibody

  • Background:

    nitric oxide synthase 1(NOS1) Homo sapiens The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms

  • Protein Name:

    Nitric oxide synthase brain

  • Human Gene Id:

    4842

  • Human Swiss Prot No:

    P29475

  • Mouse Gene Id:

    18125

  • Mouse Swiss Prot No:

    Q9Z0J4

  • Rat Gene Id:

    24598

  • Rat Swiss Prot No:

    P29476

  • Specificity:

    Phospho-NOS1 (S852) Polyclonal Antibody detects endogenous levels of NOS1 protein only when phosphorylated at S852.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    NOS1; Nitric oxide synthase; brain; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS

  • MolecularWeight(Da):

    160559

  • Observed Band(KD):

    160

  • Function:

    alternative products:Isoform 3 is produced by different alternative splicing events implicating either the untranslated exons TEX1 (TN-NOS) or TEX1B (TN-NOSB) leading to a N-terminus truncated protein which possesses enzymatic activity comparable to that of isoform 1. The C-terminal truncated isoform 4 is produced by insertion of the TEX2 exon between exons 3 and 4 of isoform 1, leading to a frameshift and a premature stop codon,catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,disease:Genetic variations in NOS1 gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1) [MIM:179010]. IHPS has an incidence of 1-5 per 1'000 live births in whites and a marked

  • Subcellular Location:

    photoreceptor inner segment,cytoplasm,mitochondrion,cytosol,cytoskeleton,caveola,sarcoplasmic reticulum,Z disc,T-tubule,sarcoplasmic reticulum membrane,sarcolemma,dendritic spine,protein complex,membrane ra

  • Expression:

    Brain,Cerebellum,Muscle,Retina,Testis,

  • Immunofluorescence analysis of HeLa cells, using n-NOS (Phospho-Ser852) Antibody. The picture on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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NOS1 (phospho Ser852) Polyclonal Antibody | EnoGene Biotech Co. Ltd