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mTOR (phospho Ser2448) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60176

  • Amount:

    100ul

  • Molecular Weight:

    288892Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human mTOR around the phosphorylation site of Ser2448. AA range:2415-2464

  • Source:

    Polyclonal, Rabbit,IgG

  • IWang, Tao, et al. "RAPTOR promotes colorectal cancer proliferation by inducing mTORC1 and upregulating ribosome assembly factor URB1." Cancer medicine 9.4 (2020): 1529-1543.

  • Gene Name:

    MTOR

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

  • Background:

    The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008],

  • Protein Name:

    Serine/threonine-protein kinase mTOR

  • Human Gene Id:

    2475

  • Human Swiss Prot No:

    P42345

  • Mouse Gene Id:

    56717

  • Mouse Swiss Prot No:

    Q9JLN9

  • Rat Gene Id:

    56718

  • Rat Swiss Prot No:

    P42346

  • Specificity:

    Phospho-mTOR (S2448) Polyclonal Antibody detects endogenous levels of mTOR protein only when phosphorylated at S2448.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic tar

  • Observed Band(KD):

    289

  • Function:

    function:Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.,similarity:Belongs to the PI3/PI4-kinase family.,similarity:Contains 1 FAT domain.,similarity:Contains 1 FATC domain.,similarity:Contains 1 PI3K/PI4K domain.,similarity:Contains 7 HEAT repeats.,subunit:Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex.,tissue specificity:Expressed in numerous tissues, with highest levels in testis.,

  • Subcellular Location:

    Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome . Cytoplasm . Nucleus, PML body . Microsome membrane . Lysosome membrane . Cytoplasmic vesicle, phagosome . Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB (PubMed:18497260, PubMed:20381137). Lysosome targeting also depends on interaction with MEAK7. Translocates to the lysosome membrane in the presence of TM4SF5 (PubMed:30956113). .

  • Expression:

    Expressed in numerous tissues, with highest levels in testis.

  • Wei, Fu-Zheng, et al. "Epigenetic regulation of autophagy by the methyltransferase EZH2 through an MTOR-dependent pathway." Autophagy 11.12 (2015): 2309-2322.

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Catalog Number

  • Amount:100ul
  • Price:225$

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mTOR (phospho Ser2448) Polyclonal Antibody | EnoGene Biotech Co. Ltd