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MEK-2 (phospho Thr394) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60169

  • Amount:

    100ul

  • Molecular Weight:

    44424Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human MEK2 around the phosphorylation site of Thr394. AA range:261-310

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-MEK-2 (T394) Polyclonal Antibody diluted at 1:2000

  • Gene Name:

    MAP2K2

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Dual specificity mitogen-activated protein kinase kinase 2

  • Human Gene Id:

    5606

  • Human Swiss Prot No:

    P36507

  • Mouse Gene Id:

    26396

  • Mouse Swiss Prot No:

    Q63932

  • Rat Gene Id:

    58960

  • Rat Swiss Prot No:

    P36506

  • Specificity:

    Phospho-MEK-2 (T394) Polyclonal Antibody detects endogenous levels of MEK-2 protein only when phosphorylated at T394.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.,function:C

  • Subcellular Location:

    Cytoplasm . Membrane ; Peripheral membrane protein . Membrane localization is probably regulated by its interaction with KSR1. .

  • Expression:

    Colon carcinoma,Epithelium,Human cerebellum,Muscle,Platelet

  • Western Blot analysis of VEC cells using Phospho-MEK-2 (T394) Polyclonal Antibody diluted at 1:2000

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