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LIMK-2 (phospho Thr505) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60162

  • Amount:

    100ul

  • Molecular Weight:

    72232Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    Synthesized phospho-peptide around the phosphorylation site of human LIMK-2 (phospho Thr505)

  • Source:

    Polyclonal, Rabbit,IgG

  • Gao, Ting-Ting, et al. "LIMK1/2 in the mPFC plays a role in chronic stress-induced depressive-like effects in mice." International Journal of Neuropsychopharmacology 23.12 (2020): 821-836.

  • Gene Name:

    LIMK2

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

  • Protein Name:

    LIM domain kinase 2

  • Human Gene Id:

    3985

  • Human Swiss Prot No:

    P53671

  • Mouse Gene Id:

    16886

  • Mouse Swiss Prot No:

    O54785

  • Rat Swiss Prot No:

    P53670

  • Specificity:

    Phospho-LIMK-2 (T505) Polyclonal Antibody detects endogenous levels of LIMK-2 protein only when phosphorylated at T505.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    LIMK2; LIM domain kinase 2; LIMK-2

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.,PTM:Phosphorylated on serine and/or threonine residues by ROCK1.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 LIM zinc-binding domains.,subcellular location:Isoform LIMK2a is distributed in the cytoplasm and the nucleus.,subcellular location:Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.,subunit:Binds ROCK1 and LKAP. Interacts with PARD3. Interacts with NISCH.,tissue specificity:Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, col

  • Subcellular Location:

    Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome .; [Isoform LIMK2a]: Cytoplasm . Nucleus .; [Isoform LIMK2b]: Cytoplasm . Cytoplasm, perinuclear region . Nucleus . Mainly present in the cytoplasm and is scarcely translocated to the nucleus. .

  • Expression:

    Hepatoma,Lung,Ovary,LIMK1/2 in the mPFC Plays a Role in Chronic Stress-Induced Depressive-Like Effects in Mice. INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY 2020 Dec 29 WB Mouse 1:500 Hippocampus ， mPFC tissue

  • Western Blot analysis of various cells using Phospho-LIMK-2 (T505) Polyclonal Antibody

  • Rat Gene Id:

    29524

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Catalog Number

  • Amount:100ul
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LIMK-2 (phospho Thr505) Polyclonal Antibody | EnoGene Biotech Co. Ltd