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Jun D (phospho Ser255) Polyclonal Antibody
Datasheet
Catalog Number:
E20-60160Amount:
100ulPrice:
225$Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human JunD around the phosphorylation site of Ser255. AA range:222-271Source:
Polyclonal, RabbitGene Name:
JUNDDilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JunD (Phospho-Ser255) Antibody. The picture on the right is blocked with the phospho peptide.
Background:
JunD proto-oncogene, AP-1 transcription factor subunit(JUND) Homo sapiens The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216). [provided by RefSeq, Nov 2013],Protein Name:
Transcription factor jun-DHuman Gene Id:
3727Human Swiss Prot No:
P17535Mouse Gene Id:
16478Mouse Swiss Prot No:
P15066Rat Gene Id:
24518Rat Swiss Prot No:
P52909Specificity:
Phospho-Jun D (S255) Polyclonal Antibody detects endogenous levels of Jun D protein only when phosphorylated at S255.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
JUND; Transcription factor jun-DMolecularWeight(Da):
35224Function:
function:Binds an AP-1 site and upon cotransfection stimulates the activity of a promoter that bears an AP-1 site.,similarity:Belongs to the bZIP family. Jun subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a dimer.,Subcellular Location:
chromatin,nuclear chromatin,nucleus,transcription factor complex,protein-DNA complex,Expression:
Epithelium,Spleen,
- Western blot analysis of lysates from 293 cells treated with Forskolin, using JunD (Phospho-Ser255) Antibody. The lane on the left is blocked with the phospho peptide.
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