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JAK1 (phospho Tyr1022) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60154

  • Amount:

    100ul

  • Molecular Weight:

    131957Da

  • Applications:

    IF/ICC;WB;IP;IHC-p;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human JAK1 around the phosphorylation site of Tyr1022. AA range:988-1037

  • Source:

    Polyclonal, Rabbit,IgG

  • Ma, J., Wei, K., Liu, J. et al. Glycogen metabolism regulates macrophage-mediated acute inflammatory responses. Nat Commun 11, 1769 (2020).

  • Gene Name:

    JAK1

  • Dilution:

    IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],

  • Protein Name:

    Tyrosine-protein kinase JAK1

  • Human Gene Id:

    3716

  • Human Swiss Prot No:

    P23458

  • Mouse Swiss Prot No:

    P52332

  • Specificity:

    Phospho-JAK1 (Y1022) Polyclonal Antibody detects endogenous levels of JAK1 protein only when phosphorylated at Y1022.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    JAK1; JAK1A; JAK1B; Tyrosine-protein kinase JAK1; Janus kinase 1; JAK-1

  • Observed Band(KD):

    132

  • Function:

    catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,domain:Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.,domain:The FERM domain mediates interaction with JAKMIP1.,function:Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.,sequence caution:Translation N-terminally extended.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.,similarity:Contains 1 FERM domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,subcellular location:Wholly intracellular, possibly membrane associated.,subunit:Interacts with IL31RA, JAKMIP1 and SHB.,tissue specif

  • Subcellular Location:

    Endomembrane system; Peripheral membrane protein. Wholly intracellular, possibly membrane associated.

  • Expression:

    Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.

  • Immunofluorescence analysis of mouse-liver tissue. 1,JAK1 (phospho Tyr1022) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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Catalog Number

  • Amount:100ul
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JAK1 (phospho Tyr1022) Polyclonal Antibody | EnoGene Biotech Co. Ltd