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IKKα (phospho Thr23) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60140

  • Amount:

    100ul

  • Molecular Weight:

    84654Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human IKK-alpha around the phosphorylation site of Thr23. AA range:15-64

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-IKKα (T23) Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    CHUK

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

  • Background:

    This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Inhibitor of nuclear factor kappa-B kinase subunit alpha

  • Human Gene Id:

    1147

  • Human Swiss Prot No:

    O15111

  • Mouse Swiss Prot No:

    Q60680

  • Specificity:

    Phospho-IKKα (T23) Polyclonal Antibody detects endogenous levels of IKKα protein only when phosphorylated at T23.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B

  • Function:

    catalytic activity:ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].,enzyme regulation:Activated when phosphorylated and inactivated when dephosphorylated.,function:Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.,PTM:Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK

  • Subcellular Location:

    Cytoplasm . Nucleus . Shuttles between the cytoplasm and the nucleus.

  • Expression:

    Widely expressed.DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway. MEDICAL ONCOLOGY Med Oncol. 2015 Apr;32(4):1-7 WB Human 5-8F-DLC-1 cell

  • Western Blot analysis of KB cells using Phospho-IKKα (T23) Polyclonal Antibody diluted at 1:1000

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