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Hrs (phospho Tyr216) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60132

  • Amount:

    100ul

  • Molecular Weight:

    86192Da

  • Applications:

    WB;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    Synthesized phospho-peptide around the phosphorylation site of human Hrs (phospho Tyr216)

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-Hrs (Y216) Polyclonal Antibody

  • Gene Name:

    HGS

  • Dilution:

    Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010],

  • Protein Name:

    Hepatocyte growth factor-regulated tyrosine kinase substrate

  • Human Gene Id:

    9146

  • Human Swiss Prot No:

    O14964

  • Mouse Gene Id:

    15239

  • Mouse Swiss Prot No:

    Q99LI8

  • Rat Gene Id:

    56084

  • Rat Swiss Prot No:

    Q9JJ50

  • Specificity:

    Phospho-Hrs (Y216) Polyclonal Antibody detects endogenous levels of Hrs protein only when phosphorylated at Y216.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    HGS; HRS; Hepatocyte growth factor-regulated tyrosine kinase substrate; Hrs; Protein pp110

  • Observed Band(KD):

    115

  • Function:

    domain:Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.,function:Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the

  • Subcellular Location:

    Cytoplasm . Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Endosome, multivesicular body membrane ; Peripheral membrane protein . Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments. .

  • Expression:

    Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.

  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Hrs (Phospho-Tyr216) Antibody

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Catalog Number

  • Amount:100ul
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Hrs (phospho Tyr216) Polyclonal Antibody | EnoGene Biotech Co. Ltd