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Histone H3 (phospho Ser10) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60129

  • Amount:

    100ul

  • Molecular Weight:

    15273Da

  • Applications:

    WB;IHC-p;IHC-f;Flow Cyt;ICC;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat;Drosophila melanogaster

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Histone H3.1 around the phosphorylation site of Ser10. AA range:1-50

  • Source:

    Polyclonal, Rabbit,IgG

  • Cao, Yuandong, et al. "Elevated TAB182 enhances the radioresistance of esophageal squamous cell carcinoma through G2‐M checkpoint modulation." Cancer Medicine 10.9 (2021): 3101-3112.

  • Gene Name:

    HIST1H3A

  • Dilution:

    Western Blot: 1/500 - 1/2000. IHC: 1/100 - 1/300. ICC/IF: 1/200 - 1/1000. ELISA: 1/10000. IHC-f,Flow Cyt 1:500

  • Background:

    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

  • Protein Name:

    Histone H3.1

  • Human Gene Id:

    8350/8351/8352/8353/8354/8355/8356/8357/8358/8968

  • Human Swiss Prot No:

    P68431/Q71DI3/P84243

  • Mouse Gene Id:

    319152/15077/15078

  • Rat Gene Id:

    291159/100361558

  • Rat Swiss Prot No:

    Q6LED0/P84245

  • Specificity:

    Phospho-Histone H3 (S10) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when phosphorylated at S10.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3

  • Observed Band(KD):

    15

  • Function:

    caution:Was originally (PubMed:2587222) thought to originate from mouse.,developmental stage:Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.,function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,mass spectrometry:Monoisotopic with N-acetylserine PubMed:16457589,miscellaneous:This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).,PTM:Acetylation is generally l

  • Subcellular Location:

    Nucleus. Chromosome.

  • Expression:

    Blood,Epithelium,Kidney,Lung,Ovary,Spleen,Uterus,

  • Immunofluorescence analysis of Hela cell. 1,Histone H3 (phospho Ser10) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-Tubulin Monoclonal Antibody(5G3)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).

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Catalog Number

  • Amount:100ul
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Histone H3 (phospho Ser10) Polyclonal Antibody | EnoGene Biotech Co. Ltd