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GSK3α/β (phospho Tyr279/216) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60123

  • Amount:

    100ul

  • Molecular Weight:

    50981Da

  • Applications:

    IF/ICC;WB;IHC-p;ELISA

  • Reactivity:

    Hman;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human GSK3 alpha/beta around the phosphorylation site of Tyr279/216. AA range:246-295

  • Source:

    Polyclonal, Rabbit,IgG

  • Immunofluorescence analysis of Hela cell. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

  • Gene Name:

    GSK3A/GSK3B

  • Dilution:

    IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

  • Background:

    glycogen synthase kinase 3 alpha(GSK3A) Homo sapiens This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011],

  • Protein Name:

    Glycogen synthase kinase-3 alpha/beta

  • Human Gene Id:

    2931

  • Human Swiss Prot No:

    P49840/P49841

  • Mouse Gene Id:

    606496/56637

  • Rat Gene Id:

    50686/84027

  • Rat Swiss Prot No:

    P18265/P18266

  • Specificity:

    Phospho-GSK3α/β (Y279/216) Polyclonal Antibody detects endogenous levels of GSK3α/β protein only when phosphorylated at Y279/216.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    GSK3A; Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B

  • Observed Band(KD):

    51,46

  • Function:

    catalytic activity:ATP + [tau protein] = ADP + [tau protein] phosphate.,function:Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer.,

  • Subcellular Location:

    mitochondrion,cytosol,beta-catenin destruction complex,postsynapse,

  • Expression:

    Brain,Eye,Foreskin,Lung,Pancreas,Platelet,Testis,

  • IImmunofluorescence analysis of mouse-liver tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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