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G3BP1 (phospho Ser232) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60117

  • Amount:

    100ul

  • Molecular Weight:

    52164Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human G3BP-1 around the phosphorylation site of Ser232. AA range:216-248

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-G3BP1 (S232) Polyclonal Antibody

  • Gene Name:

    G3BP1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

  • Background:

    This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Ras GTPase-activating protein-binding protein 1

  • Human Gene Id:

    10146

  • Human Swiss Prot No:

    Q13283

  • Mouse Gene Id:

    27041

  • Mouse Swiss Prot No:

    P97855

  • Specificity:

    Phospho-G3BP1 (S232) Polyclonal Antibody detects endogenous levels of G3BP1 protein only when phosphorylated at S232.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    G3BP1; G3BP; Ras GTPase-activating protein-binding protein 1; G3BP-1; ATP-dependent DNA helicase VIII; hDH VIII; GAP SH3 domain-binding protein 1

  • Observed Band(KD):

    52 60

  • Function:

    cofactor:Magnesium. Required for helicase activity.,domain:The NTF2 domain mediates multimerization.,function:May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.,PTM:Arg-435 is dimethylated, probably to asymmetric dimethylarginine.,PTM:Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a

  • Subcellular Location:

    Cytoplasm, cytosol . Perikaryon . Cytoplasm, Stress granule . Nucleus . Cytoplasmic in proliferating cells (PubMed:11604510). Cytosolic and partially nuclear in resting cells (PubMed:11604510). Recruited to stress granules in response to arsenite treatment (PubMed:12642610, PubMed:20180778). The unphosphorylated form is recruited to stress granules (PubMed:12642610). HRAS signaling contributes to this process by regulating G3BP dephosphorylation (PubMed:12642610). .

  • Expression:

    Ubiquitous.

  • Immunohistochemistry analysis of paraffin-embedded human breast cancer, using G3BP-1 (Phospho-Ser232) Antibody. The picture on the right is blocked with the G3BP-1 (Phospho-Ser232) peptide.

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Catalog Number

  • Amount:100ul
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G3BP1 (phospho Ser232) Polyclonal Antibody | EnoGene Biotech Co. Ltd