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eIF4E (phospho Ser209) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60094

  • Amount:

    100ul

  • Molecular Weight:

    25097Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human eIF4E around the phosphorylation site of Ser209. AA range:168-217

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-eIF4E (S209) Polyclonal Antibody

  • Gene Name:

    EIF4E

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

  • Background:

    The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],

  • Protein Name:

    Eukaryotic translation initiation factor 4E

  • Human Gene Id:

    1977

  • Human Swiss Prot No:

    P06730

  • Mouse Gene Id:

    13684

  • Mouse Swiss Prot No:

    P63073

  • Rat Gene Id:

    117045

  • Rat Swiss Prot No:

    P63074

  • Specificity:

    Phospho-eIF4E (S209) Polyclonal Antibody detects endogenous levels of eIF4E protein only when phosphorylated at S209.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

  • Observed Band(KD):

    25

  • Function:

    caution:Was originally thought to be phosphorylated on Ser-53 (PubMed:3112145); this was later shown to be wrong (PubMed:7665584).,function:Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.,PTM:Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.,similarity:Belongs to the eukaryotic initiation factor 4E family.,subunit:eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete wi

  • Subcellular Location:

    Cytoplasm, P-body . Cytoplasm . Cytoplasm, Stress granule . Nucleus . Interaction with EIF4ENIF1/4E-T is required for localization to processing bodies (P-bodies) (PubMed:16157702, PubMed:24335285, PubMed:25923732). Imported in the nucleus via interaction with EIF4ENIF1/4E-T via a piggy-back mechanism (PubMed:10856257). .

  • Expression:

    Brain,Fetal brain,Placenta,Pooled,Small intestine,Testis,

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Catalog Number

  • Amount:100ul
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eIF4E (phospho Ser209) Polyclonal Antibody | EnoGene Biotech Co. Ltd