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eIF2α (phospho Ser51) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60093

  • Amount:

    100ul

  • Molecular Weight:

    36112Da

  • Applications:

    IF/ICC;WB;IHC-p;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human eIF2 alpha around the phosphorylation site of Ser51. AA range:21-70

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of 293 cells using Phospho-eIF2α (S51) Polyclonal Antibody diluted at 1:2000

  • Gene Name:

    EIF2S1

  • Dilution:

    IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010],

  • Protein Name:

    Eukaryotic translation initiation factor 2 subunit 1

  • Human Gene Id:

    1965

  • Human Swiss Prot No:

    P05198

  • Mouse Gene Id:

    13665

  • Mouse Swiss Prot No:

    Q6ZWX6

  • Rat Gene Id:

    54318

  • Rat Swiss Prot No:

    P68101

  • Specificity:

    Phospho-eIF2α (S51) Polyclonal Antibody detects endogenous levels of eIF2α protein only when phosphorylated at S51.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    EIF2S1; EIF2A; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha

  • Observed Band(KD):

    38

  • Function:

    function:Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.,PTM:Substrate for at least 4 kinases: EIF2AK3/PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. In case of infection by vaccinia virus or rotavirus

  • Subcellular Location:

    Cytoplasm, Stress granule . Colocalizes with NANOS3 in the stress granules. .

  • Expression:

    B cells,Brain,Fibroblast,Placenta,

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eIF2α (phospho Ser51) Polyclonal Antibody | EnoGene Biotech Co. Ltd