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Cyclin B1 (phospho Ser126) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60079

  • Amount:

    100ul

  • Molecular Weight:

    48337Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Cyclin B1 around the phosphorylation site of Ser126. AA range:91-140

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-Cyclin B1 (S126) Polyclonal Antibody

  • Gene Name:

    CCNB1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

  • Background:

    The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008],

  • Protein Name:

    G2/mitotic-specific cyclin-B1

  • Human Gene Id:

    891

  • Human Swiss Prot No:

    P14635

  • Mouse Gene Id:

    268697

  • Mouse Swiss Prot No:

    P24860

  • Rat Gene Id:

    25203

  • Rat Swiss Prot No:

    P30277

  • Specificity:

    Phospho-Cyclin B1 (S126) Polyclonal Antibody detects endogenous levels of Cyclin B1 protein only when phosphorylated at S126.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CCNB1; CCNB; G2/mitotic-specific cyclin-B1

  • Observed Band(KD):

    60

  • Function:

    developmental stage:Accumulates steadily during G2 and is abruptly destroyed at mitosis.,function:Essential for the control of the cell cycle at the G2/M (mitosis) transition.,PTM:Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.,similarity:Belongs to the cyclin family.,similarity:Belongs to the cyclin family. Cyclin AB subfamily.,subunit:Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.,

  • Subcellular Location:

    Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

  • Expression:

    Breast adenocarcinoma,Lung,Placenta,

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