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Cofilin (phospho Ser3) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60070

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Cofilin around the phosphorylation site of Ser3. AA range:1-50

  • Source:

    Polyclonal, Rabbit

  • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

  • Gene Name:

    CFL1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    cofilin 1(CFL1) Homo sapiens The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004],

  • Protein Name:

    Cofilin-1

  • Human Gene Id:

    3716

  • Human Swiss Prot No:

    P23528

  • Mouse Gene Id:

    12631

  • Mouse Swiss Prot No:

    P18760

  • Rat Gene Id:

    29271

  • Rat Swiss Prot No:

    P45592

  • Specificity:

    Phospho-Cofilin (S3) Polyclonal Antibody detects endogenous levels of Cofilin protein only when phosphorylated at S3.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CFL1; CFL; Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin; non-muscle isoform

  • MolecularWeight(Da):

    18371

  • Observed Band(KD):

    19

  • Function:

    function:Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.,online information:Cofilin entry,PTM:Phosphorylated on Ser-3 in resting cells.,similarity:Belongs to the actin-binding proteins ADF family.,similarity:Contains 1 ADF-H domain.,subcellular location:Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.,tissue specificity:Widely distributed in various tissues.,

  • Subcellular Location:

    extracellular space,nucleus,cytoplasm,focal adhesion,actin cytoskeleton,membrane,nuclear matrix,extracellular matrix,lamellipodium membrane,vesicle,ruffle membrane,extracellular exosome,

  • Expression:

    B-cell lymphoma,Brain,Cajal-Retzius cell,Epithelium,Fetal brain cortex,Lung,Ovary,Placenta,Platelet

  • Western blot analysis of lysates from NIH/3T3 cells treated with paclitaxel 1uM 24hours, using Cofilin (Phospho-Ser3) Antibody. The lane on the right is blocked with the phospho peptide.

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  • Amount:100ul
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