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Cell Signal Transduction Research Antibodies
Chk1 (phospho Ser345) Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight54420Da
Protein NameSerine/threonine-protein kinase Chk1
Human Gene Id1111
Human Swiss Prot NoO14757
Mouse Gene Id12649
Mouse Swiss Prot NoO35280
Rat Gene Id140583
Rat Swiss Prot NoQ91ZN7
SpecificityPhospho-Chk1 (S345) Polyclonal Antibody detects endogenous levels of Chk1 protein only when phosphorylated at S345.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameCHEK1; CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1
Functioncatalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The autoinhibitory region (AIR) inhibits the activity of the kinase domain.,function:Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds
Subcellular Locationchromosome, telomeric region,chromatin,condensed nuclear chromosome,extracellular space,nucleus,nucleoplasm,replication fork,centrosome,cytosol,intracellular membrane-bounded organelle,
ExpressionBone marrow,Muscle,
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser345. AA range:310-359
Gene NameCHEK1
DilutionImmunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Backgroundcheckpoint kinase 1(CHEK1) Homo sapiens The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011],
Conjugated Antibody
This product is also available conjugated. Format: E20-60063-Conjugate. Price: $485 / Size: 100μl.
E20-60063-FITC$485 / 100μlE20-60063-Biotin$485 / 100μlE20-60063-HRP$485 / 100μlE20-60063-AP$485 / 100μlE20-60063-AF350$485 / 100μlE20-60063-AF405M$485 / 100μlE20-60063-AF488$485 / 100μlE20-60063-AF514$485 / 100μlE20-60063-AF532$485 / 100μlE20-60063-AF555$485 / 100μlE20-60063-AF568$485 / 100μlE20-60063-AF405S$485 / 100μlE20-60063-AF405L$485 / 100μlE20-60063-AF546$485 / 100μlE20-60063-AF594$485 / 100μlE20-60063-AF610$485 / 100μlE20-60063-AF635$485 / 100μlE20-60063-AF647$485 / 100μlE20-60063-AF680$485 / 100μlE20-60063-AF700$485 / 100μlE20-60063-AF750$485 / 100μlE20-60063-AF790$485 / 100μlE20-60063-APC$485 / 100μlE20-60063-PE$485 / 100μlE20-60063-Cy3$485 / 100μlE20-60063-Cy5$485 / 100μlE20-60063-Cy5.5$485 / 100μlE20-60063-Cy7$485 / 100μlE20-60063-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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