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Cdc25B (phospho Ser323) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60057

  • Amount:

    100ul

  • Molecular Weight:

    64987Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human CDC25B around the phosphorylation site of Ser323. AA range:289-338

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of VEC cells using Phospho-Cdc25B (S323) Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    CDC25B

  • Dilution:

    Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

  • Background:

    cell division cycle 25B(CDC25B) Homo sapiens CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008],

  • Protein Name:

    M-phase inducer phosphatase 2

  • Human Gene Id:

    994

  • Human Swiss Prot No:

    P30305

  • Mouse Gene Id:

    12531

  • Mouse Swiss Prot No:

    P30306

  • Rat Gene Id:

    171103

  • Rat Swiss Prot No:

    P48966

  • Specificity:

    Phospho-Cdc25B (S323) Polyclonal Antibody detects endogenous levels of Cdc25B protein only when phosphorylated at S323.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CDC25B; CDC25HU2; M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B

  • Observed Band(KD):

    70

  • Function:

    catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,enzyme regulation:Stimulated by B-type cyclins.,function:Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.,PTM:Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein.,similarity:Belongs to the MPI phosphatase family.,similarity:Contains 1 rhodanese domain.,

  • Subcellular Location:

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole .

  • Expression:

    Brain,Rectum tumor,

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