You are here: EnoGene Home > Products > Cell Signal Transduction Research Antibodies > C/EBP β (phospho Thr235) Polyclonal Antibody

C/EBP β (phospho Thr235) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60040

  • Amount:

    100ul

  • Molecular Weight:

    36106Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human C/EBP-beta around the phosphorylation site of Thr235/188. AA range:201-250

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-C/EBP β (T235) Polyclonal Antibody diluted at 1:500

  • Gene Name:

    CEBPB

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. [provided by RefSeq, Oct 2013],

  • Protein Name:

    CCAAT/enhancer-binding protein beta

  • Human Gene Id:

    1051

  • Human Swiss Prot No:

    P17676

  • Mouse Gene Id:

    12608

  • Mouse Swiss Prot No:

    P28033

  • Rat Gene Id:

    24253

  • Rat Swiss Prot No:

    P21272

  • Specificity:

    Phospho-C/EBP β (T235) Polyclonal Antibody detects endogenous levels of C/EBP β protein only when phosphorylated at T235.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CEBPB; LAP; TCF5; PP9092; CCAAT/enhancer-binding protein beta; C/EBP beta; Liver activator protein; Nuclear factor NF-IL6; Transcription factor 5; TCF-5

  • Observed Band(KD):

    36

  • Function:

    function:Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'.,PTM:Sumoylated by polymeric chains of SUMO2 or SUMO3.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. C/EBP subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha, delta and gamma. Interacts with TRIM28 and PTGES2.,tissue specificity:Expressed at low levels in the lung, kidney and spleen.,

  • Subcellular Location:

    Nucleus . Cytoplasm . Translocates to the nucleus when phosphorylated at Ser-288. In T-cells when sumoylated drawn to pericentric heterochromatin thereby allowing proliferation (By similarity). .

  • Expression:

    Expressed at low levels in the lung, kidney and spleen.

Add to Cart

Related Products

PTEN (Phospho-Ser380) Antibody
PTEN (Phospho-Ser380) Antibody Datasheet  

  • Catalog Number:

    E011009-1

  • Amount:

    50μg/50μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    IHC: 1:50~1:100

  • Swiss-Prot No.:

    P60484

  • Price:

    145$
  • Add to Cart
PTEN (Phospho-Ser380) Antibody
PTEN (Phospho-Ser380) Antibody Datasheet  

  • Catalog Number:

    E011009-2

  • Amount:

    100μg/100μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    IHC: 1:50~1:100

  • Swiss-Prot No.:

    P60484

  • Price:

    225$
  • Add to Cart
NFκB-p65 (Phospho-Ser468) Antibody
NFκB-p65 (Phospho-Ser468) Antibody Datasheet  

  • Catalog Number:

    E011013-1

  • Amount:

    50μg/50μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IF:1:100~1:200

  • Swiss-Prot No.:

    Q04206

  • Price:

    145$
  • Add to Cart
NFκB-p65 (Phospho-Ser468) Antibody
NFκB-p65 (Phospho-Ser468) Antibody Datasheet  

  • Catalog Number:

    E011013-2

  • Amount:

    100μg/100μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IF:1:100~1:200

  • Swiss-Prot No.:

    Q04206

  • Price:

    225$
  • Add to Cart
Rel (Phospho-Ser503) Antibody
Rel (Phospho-Ser503) Antibody Datasheet  

  • Catalog Number:

    E011020-1

  • Amount:

    50μg/50μl

  • Reactivity:

    Human

  • Applications:

    IF:1:100~1:200

  • Swiss-Prot No.:

    Q04864

  • Price:

    145$
  • Add to Cart

Catalog Number

  • Amount:100ul
  • Price:225$

Add to Cart

AbPromise

Contact us