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AMPKα1 (phospho Ser496) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60010

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human AMPK1 around the phosphorylation site of Ser496. AA range:451-500

  • Source:

    Polyclonal, Rabbit

  • Ding, Hongyan, et al. "Epigallocatechin‐3‐gallate activates the AMP‐activated protein kinase signaling pathway to reduce lipid accumulation in canine hepatocytes." Journal of Cellular Physiology 236.1 (2021): 405-416.

  • Gene Name:

    PRKAA1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

  • Background:

    protein kinase AMP-activated catalytic subunit alpha 1(PRKAA1) Homo sapiens The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

  • Protein Name:

    5'-AMP-activated protein kinase catalytic subunit alpha-1

  • Human Gene Id:

    5562

  • Human Swiss Prot No:

    Q13131

  • Mouse Gene Id:

    105787

  • Mouse Swiss Prot No:

    Q5EG47

  • Rat Gene Id:

    65248

  • Rat Swiss Prot No:

    P54645

  • Specificity:

    Phospho-AMPKα1 (S496) Polyclonal Antibody detects endogenous levels of AMPKα1 protein only when phosphorylated at S496.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1

  • MolecularWeight(Da):

    62808

  • Observed Band(KD):

    62

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-174 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.,function:Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic s

  • Subcellular Location:

    intracellular,nucleus,nucleoplasm,cytoplasm,cytosol,apical plasma membrane,nucleotide-activated protein kinase complex,

  • Expression:

    Brain,Intestine,Liver,Mammary gland,Platelet,Testis

  • Western Blot analysis of HELA cells using Phospho-AMPKα1 (S496) Polyclonal Antibody diluted at 1:2000

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Catalog Number

  • Amount:100ul
  • Price:225$

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