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Histone H2B (Tri Methyl Lys5) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-53321

  • Amount:

    100ul

  • Molecular Weight:

    14167/13950/13906Da

  • Applications:

    WB

  • Reactivity:

    Human;Mouse;RatFields:>>Neutrophil extracellular trap formation;>>Alcoholism;>>Viral carcinogenesis;>>Systemic lupus erythematosus

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

  • Immunogen:

    Synthetic Peptide of Histone H2B (Tri Methyl Lys5)

  • Source:

    Polyclonal, Rabbit,IgG

  • Western blot analysis of 1) Hela, 2) Rat Brain, 3) 3T3, diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

  • Gene Name:

    HIST1H2BC

  • Dilution:

    WB: 1:1000-2000

  • Background:

    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015],

  • Protein Name:

    Histone H2B type 1-A/Histone H2B type 1-B/Histone H2B type 1-C/E/F/G/I

  • Human Gene Id:

    255626/3018/3017/8339/8343/8344/8346/8347

  • Human Swiss Prot No:

    Q96A08/P33778/P62807

  • Mouse Gene Id:

    319177/319178/319179

  • Rat Gene Id:

    24829

  • Rat Swiss Prot No:

    Q00729

  • Specificity:

    The antibody detects endogenous Histone H2B (Tri Methyl Lys5) protein.

  • Formulation:

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    H2BK5ME3; HIST1H2BA; TSH2B; Histone H2B type 1-A; Histone H2B, testis; Testis-specific histone H2B; HIST1H2BB; H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f; HIST1H2BC; H2BFL; HIST1H2BE; H2BFH; HIST1H2BF; H2BFG; HIST1H2BG; H2BFA; HIST1H2BI; H2BFK; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l; YM3321

  • Observed Band(KD):

    14

  • Function:

    function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,PTM:Monoubiquitination of Lys-122 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.,similarity:Belongs to the histone H2B family.,subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one

  • Subcellular Location:

    Nucleus . Chromosome .

  • Expression:

    Mainly expressed in testis, and the corresponding protein is also present in mature sperm (at protein level). Also found in some fat cells.

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Catalog Number

  • Amount:100ul
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Histone H2B (Tri Methyl Lys5) Polyclonal Antibody | EnoGene Biotech Co. Ltd