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β-actin Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-53214

  • Amount:

    100ul

  • Applications:

    WB

  • Reactivity:

    Human;Mouse;Monkey;Goat;Rat;Hamster;Saccharo

  • Purification:

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    Purified recombinant human beta actin protein fragments expressed in E.coli.

  • Source:

    Monoclonal, Mouse

  • Western blot detection of beta actin in C6,3T3,COS7,CHO-k1,K562 and Goat muscle cell lysates using beta actin mouse mAb (1:10000 diluted).Predicted band size:45KDa.Observed band size:45KDa.

  • Gene Name:

    beta-actin

  • Dilution:

    wb 1:10000

  • Background:

    This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],

  • Human Gene Id:

    60

  • Human Swiss Prot No:

    P60709

  • Mouse Swiss Prot No:

    P60710

  • Specificity:

    This antibody detects endogenous levels of beta actin and does not cross-react with related proteins.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    A X actin like protein;ACTB;ACTB_HUMAN;Actin;Actin cytoplasmic 1;Actx;b actin;b-actin;Beta cytoskeletal actin;Beta-actin;cytoplasmic 1;Melanoma X actin;N-terminally processed;PS1TP5 binding protein 1;PS1TP5BP1.

  • Observed Band(KD):

    45

  • Function:

    disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.,function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the

  • Subcellular Location:

    Cytoplasm, cytoskeleton . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .

  • Expression:

    B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car

  • Western blot detection of beta actin in 5ug & 10ug S.cerevisiae and 5ug & 10ug P.pastoris cell lysates using beta actin mouse mAb (1:5000 diluted).Predicted band size:45KDa.Observed band size:45KDa.

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Catalog Number

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