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mCherry-Tag Polyclonal Antibody
Datasheet
Catalog Number:
E20-53212Amount:
100ulApplications:
WBReactivity:
Species independentPurification:
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using specific immunogen.Immunogen:
Recombinant Protein of mCherry-TagSource:
Polyclonal, Mouse
- Western blot analysis of mCherry Recombinant protein, diluted at 1) 1:5000 2) 1:20000. Secondary antibody was diluted at 1:20000
Dilution:
WB: 1:5000 -10000Background:
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.Specificity:
The antibody detects mCherry and mCherry tag fusion proteins.Formulation:
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.Storage Stability:
-20°C/1 year
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