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GST-Tag Polyclonal Antibody
Datasheet
Catalog Number:
E20-53205Amount:
100ulApplications:
WB;ELISAReactivity:
Species independentPurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.Immunogen:
Recombinant Protein of GST-TagSource:
Polyclonal, Rabbit,IgG
- 0.5ug GST fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:20000. Secondary antibody was diluted at 1:20000
Dilution:
WB: 1:5000-10000 ELISA 1:5000-20000Background:
GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells.Specificity:
The antibody detects C-terminal, internal, and N-terminal GST fusion proteins.Formulation:
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.Storage Stability:
-20°C/1 yearOther Name:
YM3205
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