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eNOS Monoclonal Antibody
Datasheet   

  • Catalog Number:

    E20-53164

  • Amount:

    100ul

  • Applications:

    WB

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

  • Immunogen:

    Recombinant Protein of eNOS

  • Source:

    Monoclonal, Mouse

  • Western blot analysis of Rat Heart Tissue, diluted at 1:1000.

  • Gene Name:

    NOS3

  • Dilution:

    WB: 1:500-2000

  • Background:

    Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],

  • Protein Name:

    Nitric oxide synthase, endothelial

  • Human Gene Id:

    4846

  • Human Swiss Prot No:

    P29474

  • Mouse Gene Id:

    18127

  • Mouse Swiss Prot No:

    P70313

  • Rat Gene Id:

    24600

  • Rat Swiss Prot No:

    Q62600

  • Specificity:

    The antibody detects endogenous eNOS protein.

  • Formulation:

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    NOS3; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

  • Observed Band(KD):

    130

  • Function:

    catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.,function:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.,online information:Nitric oxide synthase entry,polymorphism:Variation in NOS3 seem to be associated with susceptibility to coronary spasm.,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like

  • Subcellular Location:

    Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.

  • Expression:

    Platelets, placenta, liver and kidney.

  • The picture was kindly provided by our customer

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Catalog Number

  • Amount:100ul
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