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Histone H2B Monoclonal Antibody
Datasheet   

  • Catalog Number:

    E20-53162

  • Amount:

    100ul

  • Applications:

    WB;IF/ICC;

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

  • Immunogen:

    Synthetic Peptide of Histone H2B

  • Source:

    Monoclonal, Mouse

  • Western blot analysis of 1) Hela, 2) 3T3, 3) Raw264.7, 4) Rat Brain, 5) Rat Kidney, diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

  • Gene Name:

    HIST1H2BB

  • Dilution:

    WB: 1:1000-3000 IF 1:200

  • Background:

    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

  • Protein Name:

    Histone H2B type 1-B

  • Human Gene Id:

    3018

  • Human Swiss Prot No:

    P33778

  • Mouse Gene Id:

    319178

  • Mouse Swiss Prot No:

    Q64475

  • Specificity:

    The antibody detects endogenous Histone H2B protein.

  • Formulation:

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    HIST1H2BB; H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f

  • Observed Band(KD):

    14

  • Function:

    function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,PTM:Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.,PTM:Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DN

  • Subcellular Location:

    Nucleus. Chromosome.

  • Expression:

    Epithelium,

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Catalog Number

  • Amount:100ul
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Histone H2B Monoclonal Antibody | EnoGene Biotech Co. Ltd