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β II tubulin Monoclonal Antibody
Datasheet   

  • Catalog Number:

    E20-53151

  • Amount:

    100ul

  • Applications:

    WB;IHC-p;IF(paraffin section);IP

  • Reactivity:

    Mouse;Rat;(H)

  • Purification:

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

  • Immunogen:

    Synthetic Peptide of β II tubulin

  • Source:

    Monoclonal, Mouse

  • Western blot analysis of 1) Mouse Brain tissue, 2) Rat Brain tissue, diluted at 1:100000.

  • Gene Name:

    TUBB2A

  • Dilution:

    WB: 1:50000-100000 IP:1:200 IHC 1:50-300

  • Background:

    Microtubules, key participants in processes such as mitosis and intracellular transport, are composed of heterodimers of alpha- and beta-tubulins. The protein encoded by this gene is a beta-tubulin. Defects in this gene are associated with complex cortical dysplasia with other brain malformations-5. Two transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2015],

  • Protein Name:

    Tubulin beta-2A chain

  • Human Gene Id:

    7280

  • Human Swiss Prot No:

    Q13885

  • Mouse Gene Id:

    22151

  • Mouse Swiss Prot No:

    Q7TMM9

  • Rat Gene Id:

    498736

  • Rat Swiss Prot No:

    P85108

  • Specificity:

    The antibody detects endogenous β II tubulin protein.

  • Formulation:

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    Tubulin beta-2A chain

  • Observed Band(KD):

    50

  • Function:

    function:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.,similarity:Belongs to the tubulin family.,subunit:Dimer of alpha and beta chains.,

  • Subcellular Location:

    Cytoplasm, cytoskeleton .

  • Expression:

    High expression in brain, where it represents 30% of all beta-tubulins.

  • 1) Input: Mouse Brain Tissue Lysate 2) IP product: IP dilute 1: 200 Western blot analysis: primary antibody : TDY191 1: 10000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1: 5000

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Catalog Number

  • Amount:100ul
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