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EGFR Monoclonal Antibody
Datasheet   

  • Catalog Number:

    E20-53134

  • Amount:

    100ul

  • Molecular Weight:

    134277 Da

  • Applications:

    WB

  • Reactivity:

    Human

  • Purification:

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

  • Immunogen:

    Synthetic Peptide of EGFR

  • Source:

    Monoclonal, Mouse

  • Western blot analysis of 1) A431, 2) Hela, diluted at 1:2000.

  • Gene Name:

    EGFR

  • Dilution:

    WB: 1:1000-3000

  • Background:

    The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016],

  • Protein Name:

    Epidermal growth factor receptor

  • Human Gene Id:

    1956

  • Human Swiss Prot No:

    P00533

  • Mouse Gene Id:

    13649

  • Mouse Swiss Prot No:

    Q01279

  • Specificity:

    The antibody detects endogenous EGFR protein.

  • Formulation:

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

  • Observed Band(KD):

    175

  • Function:

    catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Defects in EGFR are associated with lung cancer [MIM:211980].,function:Isoform 2/truncated isoform may act as an antagonist.,function:Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin.,miscellaneous:Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.,online information:EGFR entry,PTM:Monoubiquitinated and polyubiquitinated upon EGF stimu

  • Subcellular Location:

    Cell membrane ; Single-pass type I membrane protein . Endoplasmic reticulum membrane ; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome . Endosome membrane. Nucleus . In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER (PubMed:20674546, PubMed:17909029). Endocytosed upon activation by ligand (PubMed:2790960, PubMed:17182860, PubMed:27153536, PubMed:17909029). Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF) (PubMed:20551055). .; [Isoform 2]: Secreted.

  • Expression:

    Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.

  • 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : 1:1,000 Secondary antibody: Goat anti-Mouse IgG, Heavy Chain Specific, 1:5,000

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Catalog Number

  • Amount:100ul
  • Price:225$

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EGFR Monoclonal Antibody | EnoGene Biotech Co. Ltd