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NFkB p65 Monoclonal Antibody
Datasheet   

  • Catalog Number:

    E20-53111

  • Amount:

    100ul

  • Molecular Weight:

    60219 Da

  • Applications:

    WB;IHC-p;IF/ICC;IP

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

  • Immunogen:

    Recombinant Protein of Transcription factor p65

  • Source:

    Monoclonal, Mouse

  • Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue, diluted at 1:2000.

  • Gene Name:

    RELA

  • Dilution:

    WB: 1:1000-3000 IP:1:200 IF 1:200 IHC 1:50-300

  • Background:

    NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

  • Protein Name:

    Transcription factor p65

  • Human Gene Id:

    5970

  • Human Swiss Prot No:

    Q04206

  • Mouse Gene Id:

    19697

  • Mouse Swiss Prot No:

    Q04207

  • Specificity:

    The antibody detects endogenous p65 proteins.

  • Formulation:

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

  • Observed Band(KD):

    65

  • Function:

    function:NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by in

  • Subcellular Location:

    Nucleus . Cytoplasm . Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B) (PubMed:1493333). Colocalized with DDX1 in the nucleus upon TNF-alpha induction (PubMed:19058135). Colocalizes with GFI1 in the nucleus after LPS stimulation (PubMed:20547752). Translocation to the nucleus is impaired in L.monocytogenes infection (PubMed:20855622). .

  • Expression:

    Bone,Colon,Pancreas,Placenta,

  • 1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200

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