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Histone H3 (Tri Methyl Lys9) Monoclonal Antibody(4H8)
Datasheet   

  • Catalog Number:

    E20-53109

  • Amount:

    100ul

  • Applications:

    WB

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

  • Immunogen:

    Synthetic Peptide of Histone H3 (Tri Methyl Lys9)

  • Source:

    Monoclonal, Mouse

  • Western blot analysis of Hela, diluted at 1) 1:2,000 2) 1:5,000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

  • Gene Name:

    HIST1H3A/HIST1H3B/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B

  • Dilution:

    WB 1:1000-3000

  • Background:

    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

  • Protein Name:

    Histone H3.1/Histone H3.2/Histone H3.3

  • Human Gene Id:

    8350/8351/8352/8353/8354/8355/8356/8357/8358/8968

  • Human Swiss Prot No:

    P68431/Q71DI3/P84243

  • Mouse Gene Id:

    319152/15077/15078

  • Specificity:

    The antibody detects endogenous Histone H3 (tri methyl K9) protein.

  • Formulation:

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Storage Stability:

    -15°C to -25°C/1 year(Do not lower than -25°C)

  • Other Name:

    H3K9ME3;HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/i;Histone H3/j;Histone H3/k;Histone H3/l;HIST2H3A;HIST2H3C;H3F2;H3FM;HIST2H3D;Histone H3.2;Histone H3/m;Histone H3/o;H3F3A;H3.3A;H3F3;PP781;H3F3B;H3.3B;Histone H3.3

  • Observed Band(KD):

    15kD

  • Function:

    caution:Was originally (PubMed:2587222) thought to originate from mouse.,developmental stage:Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.,function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,mass spectrometry:Monoisotopic with N-acetylserine PubMed:16457589,miscellaneous:This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).,PTM:Acetylation is generally l

  • Subcellular Location:

    Nucleus. Chromosome.

  • Expression:

    Blood,Epithelium,Kidney,Lung,Ovary,Spleen,Uterus,

  • 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : 1:1,000 Secondary antibody: Goat anti-Mouse IgG(H+L) (S001) 1:10,000

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Catalog Number

  • Amount:100ul
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Histone H3 (Tri Methyl Lys9) Monoclonal Antibody(4H8) | EnoGene Biotech Co. Ltd