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Caspase 9 Monoclonal Antibody(3-20)
Datasheet   

  • Catalog Number:

    E20-53077

  • Amount:

    100ul

  • Applications:

    WB;IHC-p;IF/ICC;IP;

  • Reactivity:

    Human;Mouse;Rat;chicken

  • Purification:

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

  • Immunogen:

    Synthetic Peptide of Caspase 9

  • Source:

    Monoclonal, Mouse

  • Immunofluorescence analysis of Hela cell. 1,eIF2α Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Caspase 9 Monoclonal Antibody(3-20)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).

  • Gene Name:

    CASP9

  • Dilution:

    WB: 1:1000-5000 IP:1:200 IF 1:200 IHC 1:50-300

  • Background:

    This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013],

  • Protein Name:

    Caspase9

  • Human Gene Id:

    842

  • Human Swiss Prot No:

    P55211

  • Specificity:

    The antibody detects endogenous Caspase 9 protein.

  • Formulation:

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6Observed Band:46KD

  • Function:

    catalytic activity:Strict requirement for an Asp residue at position P1 and with a marked preference for His at position P2. It has a preferred cleavage sequence of Leu-Gly-His-Asp-|-Xaa.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).,function:Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.,online information:Caspase-9 entry,PTM:Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.,similarity:Belongs to the peptidase C14A family.,similarity:Contains 1 CARD domain.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers

  • Subcellular Location:

    nucleus,mitochondrion,cytosol,apoptosome,

  • Expression:

    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

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Catalog Number

  • Amount:100ul
  • Price:225$

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Caspase 9 Monoclonal Antibody(3-20) | EnoGene Biotech Co. Ltd