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NSE Monoclonal Antibody(13E2)
Datasheet   

  • Catalog Number:

    E20-53066

  • Amount:

    100ul

  • Applications:

    WB;IF/ICC;IHC-p

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

  • Immunogen:

    Synthetic Peptide of NSE

  • Source:

    Monoclonal, Mouse

  • Immunofluorescence analysis of Hela cell. 1,Cdk2 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). NSE Monoclonal Antibody(13E2)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).

  • Gene Name:

    ENO2

  • Dilution:

    WB: 1:2000 IHC: 1:200 IF 1:200

  • Background:

    enolase 2(ENO2) Homo sapiens This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Gamma-enolase

  • Human Gene Id:

    2026

  • Human Swiss Prot No:

    P09104

  • Mouse Gene Id:

    13807

  • Mouse Swiss Prot No:

    P17183

  • Rat Gene Id:

    100911625

  • Rat Swiss Prot No:

    P07323

  • Specificity:

    The antibody detects endogenous NSE proteins.

  • Formulation:

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    ENO2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSEObserved Band:47KD

  • Function:

    catalytic activity:2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O.,cofactor:Magnesium. Required for catalysis and for stabilizing the dimer.,developmental stage:During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.,function:Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.,induction:Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfeldt-Jacob disease.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.,similarity:Belongs to the enolase family.,subcellular location:Can translocate to the plasma membrane

  • Subcellular Location:

    Cytoplasm . Cell membrane . Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. .

  • Expression:

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

  • Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,NSE Monoclonal Antibody(13E2) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

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