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β-Catenin Monoclonal Antibody(13C6)
Datasheet
Catalog Number:
E20-53065Amount:
100ulApplications:
WB;IHC-p;IF(paraffin section)Reactivity:
Human;Mouse;RatPurification:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.Immunogen:
Synthetic Peptide of β-CateninSource:
Monoclonal, Mouse
- Western blot analysis of lysates from 1) Hela, 2) 293T, 3) MCF7, 4) Mouse Brain tissue, 5) Rat LiverTissue cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23910)was diluted at 1:10000, 37° 1hour. （Red） Actin β Polyclonal Antibody (cat:YT0099) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23720)was diluted at 1:10000, 37° 1hour.
Gene Name:
CTNNB1 CTNNB OK/SW-cl.35 PRO2286Dilution:
WB: 1:1000 IHC: 1:200Background:
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016],Protein Name:
Catenin-β;b-catenin;Beta catenin;Beta-catenin;Cadherin associated protein;Catenin (cadherin associated protein), beta 1, 88 kDa;Catenin beta 1;Catenin beta-1;CATNB;CHBCAT;CTNB1_HUMAN;CTNNB;CTNNB1;DKFZHuman Gene Id:
1499Human Swiss Prot No:
P35222Mouse Gene Id:
12387Mouse Swiss Prot No:
Q02248Rat Gene Id:
84353Rat Swiss Prot No:
Q9WU82Specificity:
The antibody detects endogenous β-Catenin proteins.Formulation:
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
CTNNB1; CTNNB; OK/SW-cl.35; Catenin beta-1; Beta-cateninObserved Band:92KDFunction:
disease:A chromosomal rearrangement involving CTNNB1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.,disease:Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.,disease:Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.,disease:Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumSubcellular Location:
Cytoplasm . Nucleus . Cytoplasm, cytoskeleton . Cell junction, adherens junction . Cell junction . Cell membrane . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cell junction, synapse . Cytoplasm, cytoskeleton, cilium basal body . Colocalized with RAPGEF2 and TJP1 at cell-cell contacts (By similarity). Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centExpression:
Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). Expressed in breast cancer tissues (at protein level) (PubMed:29367600).
- Western blot analysis of 1) Hela, 2) 293T, 3) MCF7, 4) Mouse Brain tissue, 3) Rat LiverTissue, diluted at 1:2000.
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