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Kif 7 Monoclonal Antibody(3F8)
Datasheet
Catalog Number:
E20-53063Amount:
100ulApplications:
IHC-P;IF/ICCReactivity:
Human;Mouse;RatPurification:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.Immunogen:
Synthetic Peptide of Kif 7Source:
Monoclonal, Mouse
- Immunofluorescence analysis of Hela cell. 1,C/EBP β Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Kif 7 Monoclonal Antibody(3F8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
Gene Name:
KIF7Dilution:
WB 500-2000 1:200 IF 1:50-200Background:
This gene encodes a cilia-associated protein belonging to the kinesin family. This protein plays a role in the sonic hedgehog (SHH) signaling pathway through the regulation of GLI transcription factors. It functions as a negative regulator of the SHH pathway by preventing inappropriate activation of GLI2 in the absence of ligand, and as a positive regulator by preventing the processing of GLI3 into its repressor form. Mutations in this gene have been associated with various ciliopathies. [provided by RefSeq, Oct 2011],Protein Name:
Kinesin-like protein KIF7Human Gene Id:
374654Human Swiss Prot No:
Q2M1P5Mouse Gene Id:
16576Mouse Swiss Prot No:
B7ZNG0Specificity:
The antibody detects endogenous Kif 7 proteins.Formulation:
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.Storage Stability:
-20°C/1 yearOther Name:
Kinesin-like protein KIF7Function:
similarity:Belongs to the kinesin-like protein family. KIF27 subfamily.,similarity:Contains 1 kinesin-motor domain.,tissue specificity:Embryonic stem cells, melanotic melanoma and Jurkat T-cells.,Subcellular Location:
Cell projection, cilium . Cytoplasm, cytoskeleton, cilium basal body . Localizes to the cilium tip.Expression:
Embryonic stem cells, melanotic melanoma and Jurkat T-cells. Expressed in heart, lung, liver, kidney, testis, retina, placenta, pancreas, colon, small intestin, prostate and thymus.
- Immunofluorescence analysis of Mouse-colon tissue. 1,Kif 7 Monoclonal Antibody(3F8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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