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Aquaporin 4 Monoclonal Antibody(4H1)
Datasheet   

  • Catalog Number:

    E20-53062

  • Amount:

    100ul

  • Applications:

    WB;IHC-p;IF/ICC

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

  • Immunogen:

    Synthetic Peptide of Aquaporin 4

  • Source:

    Monoclonal, Mouse

  • Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Aquaporin 4 Monoclonal Antibody(4H1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

  • Gene Name:

    AQP4

  • Dilution:

    WB: 1:1000 IF: 1:100-200 IHC 1:50-300

  • Background:

    This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015],

  • Protein Name:

    Aquaporin-4

  • Human Gene Id:

    361

  • Human Swiss Prot No:

    P55087

  • Mouse Gene Id:

    11829

  • Mouse Swiss Prot No:

    P55088

  • Rat Gene Id:

    25293

  • Rat Swiss Prot No:

    P47863

  • Specificity:

    The antibody detects endogenous Aquaporin 4 proteins.

  • Formulation:

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    AQP4; Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4

  • Observed Band(KD):

    48

  • Function:

    domain:Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).,function:Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.,similarity:Belongs to the MIP/aquaporin (TC 1.A.8) family.,tissue specificity:Brain - muscle >> heart, kidney, lung, and trachea.,

  • Subcellular Location:

    Cell membrane ; Multi-pass membrane protein . Basolateral cell membrane ; Multi-pass membrane protein . Endosome membrane . Cell membrane, sarcolemma ; Multi-pass membrane protein . Cell projection . Activation of the vasopressin receptor AVPR1A triggers AQP4 phosphorylation at Ser-180 and promotes its internalization from the cell membrane. Detected on brain astrocyte processes and astrocyte endfeet close to capillaries. .

  • Expression:

    Detected in skeletal muscle (PubMed:29055082). Detected in stomach, along the glandular base region of the fundic gland (at protein level) (PubMed:8601457). Detected in brain, lung and skeletal muscle, and at much lower levels in heart and ovary (PubMed:7559426, PubMed:8601457).

  • Immunofluorescence analysis of Human-appendix tissue. 1,Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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Catalog Number

  • Amount:100ul
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Aquaporin 4 Monoclonal Antibody(4H1) | EnoGene Biotech Co. Ltd