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CD20 Monoclonal Antibody(2F4)
Datasheet   

  • Catalog Number:

    E20-53048

  • Amount:

    100ul

  • Applications:

    IHC-p;IF(paraffin section)

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

  • Immunogen:

    Synthetic Peptide of CD20

  • Source:

    Monoclonal, Mouse

  • Immunohistochemical analysis of paraffin-embedded Human-breast tissue. 1,CD20 Monoclonal Antibody(2F4) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

  • Gene Name:

    MS4A1

  • Dilution:

    IHC: 1:200

  • Background:

    This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008],

  • Protein Name:

    B-lymphocyte antigen CD20

  • Human Gene Id:

    931

  • Human Swiss Prot No:

    P11836

  • Mouse Gene Id:

    12482

  • Mouse Swiss Prot No:

    P19437

  • Specificity:

    The antibody detects endogenous CD20 proteins.

  • Formulation:

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MS4A1; CD20; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD20

  • Observed Band(KD):

    35-37

  • Function:

    caution:Epitope 1, mapped in PubMed:16785532, is predicted to be buried in the membrane. Its accessibility to the extracellular space, and thus to antibody recognition, is not explained.,function:This protein may be involved in the regulation of B-cell activation and proliferation.,online information:CD20 entry,pharmaceutical:Monoclonal antibodies(mAb) against CD20 are used to treat B-cell non-Hodgkin's lymphoma (NHL). These antibodies include Rituximab (Mabthera), Britumomab (Zevalin) and Tositumomab (Bexxar). CD20 engaged by mAb can generate transmembrane signals capable of directly controlling cell growth and triggering cell death in certain tumors. Alternatively, mAb can mediate complement-dependent cytotoxicity.,PTM:Phosphorylated. Might be functionally regulated by protein kinase(s).,similarity:Belongs to the MS4A family.,tissue specificity:Expressed on B-cells.,

  • Subcellular Location:

    Cell membrane ; Multi-pass membrane protein . Cell membrane ; Lipid-anchor . Constitutively associated with membrane rafts. .

  • Expression:

    Expressed on B-cells.

  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,CD20 Monoclonal Antibody(2F4) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

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