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HSP70 Monoclonal Antibody(3G10)
Datasheet   

  • Catalog Number:

    E20-53042
  • Amount:

    100ul
  • Applications:

    WB;IHC-p;IF/ICC
  • Reactivity:

    Human;Mouse;Rat
  • Purification:

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
  • Immunogen:

    Synthetic Peptide of HSP70
  • Source:

    Monoclonal, Mouse
  • Niu, Ziru, et al. "Polymer-based precipitation preserves biological activities of extracellular vesicles from an endometrial cell line." PloS one 12.10 (2017): e0186534.
  • Gene Name:

    HSPA1L/HSPA1A
  • Dilution:

    WB: 1:1000-2000 IF: 1:100-200 IHC 1:50-300
  • Background:

    This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq, Jul 2008],
  • Protein Name:

    Heat shock 70 kDa protein 1-like/Heat shock 70 kDa protein 1A/1B
  • Human Gene Id:

    3305/3303/3304
  • Human Swiss Prot No:

    P34931/P08107
  • Mouse Gene Id:

    15482/193740
  • Rat Gene Id:

    24963/24472
  • Rat Swiss Prot No:

    P55063/Q07439
  • Specificity:

    The antibody detects endogenous HSP70 proteins.
  • Formulation:

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
  • Storage Stability:

    -20°C/1 year
  • Observed Band(KD):

    70
  • Function:

    function:In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.,induction:Not induced by heat shock.,similarity:Belongs to the heat shock protein 70 family.,tissue specificity:Expressed in spermatids.,
  • Subcellular Location:

    zona pellucida receptor complex,nucleoplasm,mitochondrial matrix,cytosol,COP9 signalosome,cell body,blood microparticle,
  • Expression:

    Expressed in spermatids.
  • Immunofluorescence analysis of Hela cell. 1,AIM2 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). HSP70 Monoclonal Antibody(3G10)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).

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Catalog Number

  • Amount:100ul
  • Price:225$

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