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β I tubulin Monoclonal Antibody(3F7)
Datasheet   

  • Catalog Number:

    E20-53037
  • Amount:

    100ul
  • Applications:

    WB;IHC-p;IF/ICC;
  • Reactivity:

    Human;Rat;Mouse
  • Purification:

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
  • Immunogen:

    Synthetic Peptide of β I tubulin
  • Source:

    Monoclonal, Mouse
  • Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,β I tubulin Monoclonal Antibody(3F7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Gene Name:

    TUBB1
  • Dilution:

    WB: 1:500-10000 IF 1:200 IHC 1:50-300
  • Background:

    This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.[provided by RefSeq, Jul 2010],
  • Protein Name:

    Tubulin beta-1 chain
  • Human Gene Id:

    81027
  • Human Swiss Prot No:

    Q9H4B7
  • Mouse Gene Id:

    545486
  • Mouse Swiss Prot No:

    A2AQ07
  • Specificity:

    The antibody detects endgenousβ I tubulin proteins.
  • Formulation:

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
  • Storage Stability:

    -20°C/1 year
  • Other Name:

    TUBB1; Tubulin beta-1 chain; TUBB2A; TUBB2; Tubulin beta-2A chain; TUBB2B; Tubulin beta-2B chain; TUBB4B; TUBB2C; Tubulin beta-4B chain; Tubulin beta-2 chain; Tubulin beta-2C chain; TUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; TUBB4A; TUBB4; TUBB5; Tubulin beta-4A chain; Tubulin 5 beta; Tubulin beta-4 chain; TUBB; TUBB5; OK/SW-cl.56; Tubulin beta chain; Tubulin beta-5 chain; TUBB6; Tubulin beta-6 chain; TUBB8; Tubulin beta-8 chain
  • Observed Band(KD):

    50
  • Function:

    function:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.,similarity:Belongs to the tubulin family.,subunit:Dimer of alpha and beta chains.,
  • Subcellular Location:

    Cytoplasm, cytoskeleton .
  • Expression:

    Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins.
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,β I tubulin Monoclonal Antibody(3F7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

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Catalog Number

  • Amount:100ul
  • Price:225$

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β I tubulin Monoclonal Antibody(3F7) | EnoGene Biotech Co. Ltd