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Plant actin Monoclonal Antibody
Datasheet
Catalog Number:
E20-53034Amount:
100ulApplications:
WBReactivity:
VariousPlantsPurification:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.Immunogen:
Recombinant Protein of Plant actinSource:
Monoclonal, Mouse
- Western blot analysis of Arabidopsis, (Q30) diluted at 1:5000
Dilution:
WB: 1:2000-5000Background:
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques.Specificity:
The antibody detects endogenous plant actin protein.Formulation:
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.Storage Stability:
-20°C/1 yearObserved Band(KD):
43
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