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β-actin Monoclonal Antibody
Datasheet
Catalog Number:
E20-53028Amount:
100ulMolecular Weight:
41737DaApplications:
IF/ICC;WB;IHC-pReactivity:
Human;Rat;Mouse;Mk;Dg;Ch;Hamster;Rabbit;InsectPurification:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.Immunogen:
Synthetic Peptide of β-actinSource:
Monoclonal, Mouse
- Western blot analysis of Hela (1) Rat brain (2) Mouse brain (3) Chicken lung (4) and Rabbit testis (5), diluted at 1:10000.
Gene Name:
ACTBDilution:
IF: 1:50-200 WB: 1:5000-20000 IHC: 1:200-300Background:
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],Protein Name:
Actin cytoplasmic 1Human Gene Id:
60Human Swiss Prot No:
P60709Mouse Gene Id:
11461Mouse Swiss Prot No:
P60710Rat Gene Id:
81822Rat Swiss Prot No:
P60711Specificity:
The antibody detects endogenous β-actin protein.Formulation:
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β;β-Actin;Beta actinObserved Band(KD):
43Function:
disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.,function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to theSubcellular Location:
Cytoplasm, cytoskeleton . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .Expression:
B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car
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