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His-Tag Monoclonal Antibody
Datasheet
Catalog Number:
E20-53023Amount:
100ulApplications:
WBReactivity:
Species independentPurification:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.Immunogen:
Synthetic peptide:HHHHHH conjugated to KLH.Source:
Monoclonal, Mouse
- Western blot analysis of His recombinant protein, diluted at 1) 1:10000 2) 1:20000
Gene Name:
His-TagDilution:
WB: 1:3000-10000Background:
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.Protein Name:
His TagSpecificity:
The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.Formulation:
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.Storage Stability:
-20°C/1 year
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