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Flag-Tag Monoclonal Antibody
Datasheet
Catalog Number:
E20-53001Amount:
100ulApplications:
WB;IP;IF/ICCReactivity:
Species independentPurification:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.Immunogen:
Synthetic Peptide of Flag-TagSource:
Monoclonal, Mouse
- 1ug Flag fusion protein+ Primary antibody dilution at 1:10000
Gene Name:
Flag TagDilution:
WB: 1:5000 IP: 1:200 IF: 1:2000Background:
The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.Protein Name:
Flag-Tag DDDDK TAG, DDDDK-TAGSpecificity:
The antibody detects C-terminal, internal, and N-terminal Flag-tag fusion proteins.Formulation:
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.Storage Stability:
-20°C/1 year
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