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NF-E4 (Acetyl Lys43) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-40049

  • Amount:

    100ul

  • Molecular Weight:

    19019Da

  • Applications:

    WB;ELISA;IHC-p

  • Reactivity:

    Human;Rat;Mouse;

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    Synthesized acetyl-peptide derived from the human NF-E4 around the acetylation site of K43.

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of AD-293 cells using Acetyl-NF-E4 (K43) Polyclonal Antibody. Secondary antibody was diluted at 1:20000

  • Gene Name:

    NFE4

  • Dilution:

    WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000

  • Background:

    The erythroid-specific protein encoded by this gene, and the ubiquitous transcription factor CP2, form the stage selector protein (SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform (22 kDa) acts as an activator, the short isoform (14 kDa) has been shown to repress gamma-globin gene expression. This gene is located in an intron of the FBXL13 gene on the opposite strand. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Transcription factor NF-E4

  • Human Gene Id:

    58160

  • Human Swiss Prot No:

    Q86UQ8

  • Specificity:

    Acetyl-NF-E4 (K43) Polyclonal AntibodySynthesized peptide derived from the human NF-E4 around the acetylation site of K43.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    NFE4; Transcription factor NF-E4

  • Observed Band(KD):

    19

  • Function:

    function:Functions as part of the SSP (stage selector protein) complex, a complex that contributes to the preferential expression of the gamma-gene in fetal erythroid cells by facilitating the interaction of the gamma-globin genes with enhancer elements contained in the locus control region (LCR). The complex binds to the stage selector element (SSE) in the proximal gamma-globin promoter. In contrast, isoform 2 acts as a repressor of gamma-globin gene expression by preventing NFE2 and RNA polymerase II recruitment to the promoter.,PTM:Acetylation at Lys-43 prolongs the protein half-life by preventing ubiquitin-mediated degradation and reduces the interaction between NF-E4 and HDAC1, potentially maximizing the activating ability of the factor at the gamma-promoter.,PTM:Ubiquitinated; leading to its degradation by the proteasome. Acetylation at Lys-43 prevents ubiquitination.,sequence caut

  • Subcellular Location:

    Nucleus .

  • Expression:

    Specifically expressed in fetal liver, cord blood and bone marrow. Also expressed in the K562 and HEL cell lines, which constitutively express the fetal globin genes.

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Catalog Number

  • Amount:100ul
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NF-E4 (Acetyl Lys43) Polyclonal Antibody | EnoGene Biotech Co. Ltd