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Cleaved-PARP-1 (G215) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-10073

  • Amount:

    100ul

  • Applications:

    WB;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human PARP. AA range:196-245

  • Source:

    Polyclonal, Rabbit,IgG

  • Wang, Bin, et al. "Loss of Tctn3 causes neuronal apoptosis and neural tube defects in mice." Cell death & disease 9.5 (2018): 520.

  • Gene Name:

    PARP1

  • Dilution:

    Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

  • Background:

    This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Poly [ADP-ribose] polymerase 1

  • Human Gene Id:

    142

  • Human Swiss Prot No:

    P09874

  • Mouse Swiss Prot No:

    P11103

  • Specificity:

    Cleaved-PARP-1 (G215) Polyclonal Antibody detects endogenous levels of fragment of activated PARP-1 protein resulting from cleavage adjacent to G215.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1

  • Observed Band(KD):

    89

  • Function:

    catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,function:Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.,miscellaneous:The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.,PTM:Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Poly-ADP-ribosylated by PARP2.,similarity:Contains 1 BRCT

  • Subcellular Location:

    Nucleus . Nucleus, nucleolus . Chromosome . Localizes to sites of DNA damage. .

  • Expression:

    Brain,Colon carcinoma,Fibroblast,Lung,Ovarian carcinoma,Skin,The role of SIRT1 in BMP2-induced chondrogenic differentiation and cartilage maintenance under oxidative stress.. Aging-US Aging-Us. 2020 May 31; 12(10): 9000–9013 WB Mouse 1:500 C3H10T1/2 cells

  • Mao, Dongwei, et al. "RNAi-mediated knockdown of the CLN3 gene inhibits proliferation and promotes apoptosis in drug-resistant ovarian cancer cells." Molecular medicine reports12.5 (2015): 6635-6641.

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