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Cleaved-Notch 4 (V1432) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-10071

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human NOTCH4. AA range:1401-1450

  • Source:

    Polyclonal, Rabbit

  • Western Blot analysis of A549 NIH-3T3 SH-SY5Y K562 HELA cells using Cleaved-Notch 4 (V1432) Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    NOTCH4

  • Dilution:

    Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

  • Background:

    notch 4(NOTCH4) Homo sapiens This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which

  • Protein Name:

    Neurogenic locus notch homolog protein 4

  • Human Gene Id:

    4855

  • Human Swiss Prot No:

    Q99466

  • Mouse Swiss Prot No:

    P31695

  • Specificity:

    Cleaved-Notch 4 (V1432) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 4 protein resulting from cleavage adjacent to V1432.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    NOTCH4; INT3; Neurogenic locus notch homolog protein 4; Notch 4; hNotch4

  • MolecularWeight(Da):

    209622

  • Observed Band(KD):

    59

  • Function:

    alternative products:Experimental confirmation may be lacking for some isoforms,function:Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.,polymorphism:The poly-Leu region of NOTCH4 (in the signal peptide) is polymorphic and the number of Leu varies in the population (from 6 to 12).,PTM:Phosphorylated.,PTM:Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma

  • Subcellular Location:

    Golgi membrane,extracellular region,nucleus,nucleoplasm,endoplasmic reticulum membrane,cytosol,plasma membrane,integral component of plasma membrane,cell surface,integral component of membrane,

  • Expression:

    Bone marrow,Heart,Peripheral Nervous System,Placenta,

  • Western blot analysis of lysates from 293 and COS7 cells, treated with etoposide 25uM 1h, using NOTCH4 (Cleaved-Val1432) Antibody. The lane on the right is blocked with the synthesized peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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Cleaved-Notch 4 (V1432) Polyclonal Antibody | EnoGene Biotech Co. Ltd