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Cleaved-Notch 2 (D1733) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-10069

  • Amount:

    100ul

  • Applications:

    IF/ICC;WB;IHC-p;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Notch 2. AA range:1684-1733

  • Source:

    Polyclonal, Rabbit,IgG

  • Pu, Y., Song, Y., Zhang, M. et al. GOLM1 restricts colitis and colon tumorigenesis by ensuring Notch signaling equilibrium in intestinal homeostasis. Sig Transduct Target Ther 6, 148 (2021).

  • Gene Name:

    NOTCH2

  • Dilution:

    IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

  • Background:

    notch 2(NOTCH2) Homo sapiens This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cle

  • Protein Name:

    Neurogenic locus notch homolog protein 2

  • Human Gene Id:

    4853

  • Human Swiss Prot No:

    Q04721

  • Mouse Gene Id:

    18129

  • Mouse Swiss Prot No:

    O35516

  • Rat Gene Id:

    29492

  • Rat Swiss Prot No:

    Q9QW30

  • Specificity:

    Cleaved-Notch 2 (D1733) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 2 protein resulting from cleavage adjacent to D1733.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    NOTCH2; Neurogenic locus notch homolog protein 2; Notch 2; hN2Observed Band:110(cleaved)KD

  • Function:

    disease:Defects in NOTCH2 are the cause of Alagille syndrome type 2 (ALGS2) [MIM:610205]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.,function:Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.,PTM:Phosphorylated.,PTM:Synthesized in the endoplasmic reticulum as an inactive form which

  • Subcellular Location:

    [Notch 2 extracellular truncation]: Cell membrane ; Single-pass type I membrane protein .; [Notch 2 intracellular domain]: Nucleus . Cytoplasm . Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by TCIM (PubMed:25985737). .

  • Expression:

    Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.

  • Ylivinkka, Irene, et al. "Motility of glioblastoma cells is driven by netrin-1 induced gain of stemness." Journal of Experimental & Clinical Cancer Research 36.1 (2017): 9.

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Catalog Number

  • Amount:100ul
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Cleaved-Notch 2 (D1733) Polyclonal Antibody | EnoGene Biotech Co. Ltd