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Cleaved-Bad (D71) Polyclonal Antibody
Datasheet
Catalog Number:
E20-10043Amount:
100ulApplications:
WB;IHC-p;IF(paraffin section);ELISAReactivity:
Mouse;RatPurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from mouse BAD. AA range:21-70Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using Cleaved-Bad (D71) Polyclonal Antibody
Gene Name:
BADDilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.Background:
BCL2 associated agonist of cell death encoded by BAD is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. BCL2 associated agonist of cell death positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of BCL2 associated agonist of cell death is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of BCL2 associated agonist of cell death. Alternative splicing of BAD results in two transcript variants which encode the same isoform.Protein Name:
Bcl2 antagonist of cell deathMouse Gene Id:
12015Rat Gene Id:
64639Rat Swiss Prot No:
O35147Specificity:
Cleaved-Bad (D71) Polyclonal Antibody detects endogenous levels of fragment of activated Bad protein resulting from cleavage adjacent to D71.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
BAD; BBC6; BCL2L8; Bcl2 antagonist of cell death; BAD; Bcl-2-binding component 6; Bcl-2-like protein 8; Bcl2-L-8; Bcl-XL/Bcl-2-associated death promoterObserved Band:20KD
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BAD (Cleaved-Asp71) Antibody. The picture on the right is blocked with the synthesized peptide.
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