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Cleaved-Caspase-4 p20 (Q81) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-10028

  • Amount:

    100ul

  • Molecular Weight:

    43262Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Caspase 4. AA range:62-111

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of 3T3 cells using Cleaved-Caspase-4 p20 (Q81) Polyclonal Antibody diluted at 1:2000
    .

  • Gene Name:

    CASP4

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

  • Background:

    This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Caspase4

  • Human Gene Id:

    837

  • Human Swiss Prot No:

    P49662

  • Mouse Swiss Prot No:

    P70343

  • Specificity:

    Cleaved-Caspase-4 p20 (Q81) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-4 p20 protein resulting from cleavage adjacent to Q81.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CASP4; ICH2; Caspase-4; CASP-4; ICE(rel)-II; Protease ICH-2; Protease TX

  • Observed Band(KD):

    43+22

  • Function:

    catalytic activity:Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but also cleaves at Asp-Glu-Val-Asp-|-.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1.,PTM:The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8.,similarity:Belongs to the peptidase C14A family.,similarity:Contains 1 CARD domain.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit.,tissue specificity:Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.,

  • Subcellular Location:

    Cytoplasm, cytosol . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion . Inflammasome . Secreted . Predominantly localizes to the endoplasmic reticulum (ER). Association with the ER membrane requires TMEM214 (PubMed:15123740). Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630). .

  • Expression:

    Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level). Not detected in brain.

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Catalog Number

  • Amount:100ul
  • Price:225$

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Cleaved-Caspase-4 p20 (Q81) Polyclonal Antibody | EnoGene Biotech Co. Ltd