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Cleaved-Caspase-9 (D353) Polyclonal Antibody
Datasheet
Catalog Number:
E20-10012Amount:
100ulApplications:
IF/ICC;WB;IHC-p;ELISAReactivity:
Human;Mouse;RatPurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human Caspase 9. AA range:323-372Source:
Polyclonal, Rabbit,IgG
- Western blot analysis of lysates from NIH/3T3 cells, treated with Etoposide 25uM 60', using Caspase 9 (Cleaved-Asp353) Antibody. The lane on the right is blocked with the synthesized peptide.
. Gene Name:
CASP9Dilution:
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.Background:
CASP9 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase 9 can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. Caspase 9 is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.Protein Name:
Caspase9Rat Gene Id:
58918Rat Swiss Prot No:
Q9JHK1Specificity:
Cleaved-Caspase-9 (D353) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-9 protein resulting from cleavage adjacent to D353.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6Observed Band(KD):
17kD
- Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 9 (Cleaved-Asp353) Antibody. The picture on the right is blocked with the synthesized peptide.
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